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Detecting disease-associated target nucleic acids in a mammal and treatment thereof
Robert Cooper, Jeff Hasty, Daniel Lindsay Worthley, Susan Lesley Woods, Josephine Wright. South Australian Health & Medical Research Institute, University of California San Diego UCSD.Detecting disease-associated target nucleic acids in a mammal and treatment thereof
Disclosed herein are methods of detecting a target nucleic acid in a sample (e.g., biological sample or environmental sample). The method includes administering to the sample an effective amount of a composition comprising an engineered bacterium (e.g., A. baylyi) and detecting an output signal. In some cases, the mammal has a cancer or an infectious disease.
US20250027168A1Methods for generating a crispr array
Robert Cooper, Jeff Hasty. University of California San Diego UCSD.Methods for generating a crispr array
Provided herein are methods for generating multiplex CRISPR arrays based on annealing and ligating single-stranded DNA oligonucleotides using bridge oligonucleotides. The methods described herein include providing a first oligonucleotide comprising a CRISPR repeat sequence or a portion thereof, and a first portion of a first spacer sequence at its 3′ end; providing a second oligonucleotide comprising, from 5′ to 3′, a second portion of the first spacer sequence, the CRISPR repeat sequence, and a first portion of a second spacer sequence; providing a bridge oligonucleotide comprising a sequence substantially complementary to the first spacer sequence; allowing the first oligonucleotide and the second oligonucleotide to hybridize with the bridge oligonucleotide; and ligating the first and second oligonucleotide.
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